Study record
CALERIE Phase 2 DunedinPACE epigenetic sub-analysis
DunedinPACE (pace-of-aging epigenetic marker)
Human — Healthy, non-obese adults, CALERIE Phase 2 RCT (DunedinPACE sub-analysis) Tier 3 · Human biomarker RCT verification: verified
1. Species / populationHealthy non-obese adult humans, CALERIE Phase 2 RCT
2. Exposure, route, scheduleCaloric restriction, 25% assigned target / ~11.9% mean achieved, 2 years
3. Comparator & durationAd libitum control diet, 2 years
4. Endpoint & numeric resultDunedinPACE reduced ~2-3%; PhenoAge/GrimAge unchanged
5. What this study did NOT establishNo lifespan data; single-clock result, not corroborated by two other clocks in the same trial
Full record
| Intervention(s) | Caloric Restriction (CR) |
| Species | Human |
| Population | Healthy, non-obese adults, CALERIE Phase 2 RCT (DunedinPACE sub-analysis) |
| Sample size | not specified in source participants verification: verified_2026-07-11 |
| Exposure | 25% assigned target / ~11.9% mean achieved % caloric reduction from baseline — Sustained over 2 years (same CALERIE Phase 2 exposure) verification: verified_2026-07-11 |
| Comparator | Ad libitum control diet |
| Duration | 2 years |
| Endpoint | DunedinPACE (pace-of-aging epigenetic marker) |
| Evidence tier | Tier 3 · Human biomarker RCT |
| What was NOT established | Did not test lifespan. A single epigenetic clock's change does not establish a generalized 'slowed aging' effect, since two other clocks tested in the same trial showed no effect; DunedinPACE is not a validated lifespan-prediction endpoint in this dataset. |
Result values (as reported in sourcing brief)
| dunedinpace change | -2% to -3% relative to control |
| phenoage grimage effect | No significant effect (same trial, different clocks) |
| interpretation | DunedinPACE reduced; other clocks (PhenoAge, GrimAge) did not show a significant change |
| verification | verification: verified_2026-07-11 |
Source citation
Verification status: verified This record was checked against its primary publication on 2026-07-11.Identifiers: PMID verified; DOI verified; PMC verified; trial registry ID not reported. Record-level verification and identifier-level status are separate: a record can be fully verified against its primary publication while an identifier that does not apply to the study type — a trial registry ID for an animal study, for instance — is marked not applicable. No identifier is fabricated, and none is left in a “pending” state.
| trial name | CALERIE Phase 2 (DunedinPACE sub-analysis) |
| pmid | 37118425 verified |
| doi | 10.1038/s43587-022-00357-y verified |
| journal | Nature Aging 2023 |
| trial registry id | not reported not reported |
| note | Author Correction exists (s43587-023-00432-y); cite main article + correction. |
| pmc | PMC10148951 verified |
| authors | Waziry R, Ryan CP, Corcoran DL, et al. |
| year | 2023 |
| verified date | 2026-07-11 |
| verified note | ~220 adults; CR slowed DunedinPACE (pace-of-aging DNAm); NO significant change in PhenoAge or GrimAge clocks — MATCHES. Modest single-algorithm effect; NOT observed life-years. |
| verification status | verified |
Funding / conflict of interest
not compiled in this proof-of-concept verification: verified_2026-07-11
Version 0.2.0-mvp · literature search date 2026-07-11 · editorial owner Nabus Research