Human — Untrained men, n=6, crossover design   Tier 8 · Human acute mechanistic verification: verified
1. Species / populationUntrained men, n=6, crossover mechanistic study
2. Exposure, route, scheduleSingle bout of exercise, crossover design
3. Comparator & durationWithin-subject crossover comparator; acute time frame
4. Endpoint & numeric resultAcute mTORC1 pathway activation observed in skeletal muscle
5. What this study did NOT establishNo lifespan/aging-rate claim; modality (aerobic vs resistance) not clearly distinguished in source
Intervention(s)Aerobic Exercise, Resistance Exercise
SpeciesHuman
PopulationUntrained men, n=6, crossover design
Sample size6 untrained men verification: verified_2026-07-11
Exposure single bout of exercise (crossover) — Acute, single-session; specific modality/intensity not further detailed in sourcing brief verification: verified_2026-07-11
ComparatorWithin-subject crossover comparator condition
DurationAcute (single-session) time frame
EndpointmTORC1 pathway activation (e.g. p-S6K1 Thr389) in skeletal muscle
Evidence tierTier 8 · Human acute mechanistic
What was NOT establishedn=6 crossover mechanistic study. Acute, muscle-localized mTORC1 activation does NOT establish any claim about organism-level aging rate, body-wide autophagy status, or lifespan. Does not establish a difference between aerobic and resistance modality (both linked to this record pending clearer modality detail in the source).
interpretationAcute activation of mTORC1 signaling observed in skeletal muscle following the exercise bout
verificationverification: verified_2026-07-11
Verification status: verified This record was checked against its primary publication on 2026-07-11.Identifiers: PMID not reported; DOI verified; PMC verified; trial registry ID not reported. Record-level verification and identifier-level status are separate: a record can be fully verified against its primary publication while an identifier that does not apply to the study type — a trial registry ID for an animal study, for instance — is marked not applicable. No identifier is fabricated, and none is left in a “pending” state.
trial nameExercise-mTOR mechanistic crossover study
pmidnot reported not reported
doi10.14814/phy2.15633 verified
journalPhysiological Reports 2023
trial registry idnot reported not reported
pmcPMC10150453 verified
authors(acute aerobic vs resistance exercise, untrained men)
year2023
verified date2026-07-11
verified note6 untrained men (27±3), crossover: 40 min cycling @70% HRmax + resistance 8x10 @65% 1RM; both raised p-mTOR Ser2448 + p-S6K1 Thr389 at 4 h; resistance greater at S6K1 Thr389 (1h) & 4E-BP1 — MATCHES. Pathway activation in ONE tissue post-exercise ≠ organismal aging.
pmid notePMID not confirmed (PMC10150453 + DOI verified).
verification statusverified

Funding / conflict of interest

not compiled in this proof-of-concept verification: verified_2026-07-11

Version 0.2.0-mvp · literature search date 2026-07-11 · editorial owner Nabus Research