Study record
Dietary metformin mouse lifespan dose-response study
Median lifespan, dose-dependent
Mus musculus — Mice, dietary metformin dosing study Tier 6 · Single-lab animal lifespan verification: verified
1. Species / populationMice, dietary metformin dosing study
2. Exposure, route, schedule0.1% metformin in diet (lifespan-extending) vs 1% (toxic), chronic
3. Comparator & durationControl diet; lifespan follow-up
4. Endpoint & numeric result+~5.83% median lifespan (male) at 0.1%; 1% dose toxic
5. What this study did NOT establishNo human data; no safe human dose established; dose-response is non-monotonic
Full record
| Intervention(s) | Metformin |
| Species | Mus musculus |
| Population | Mice, dietary metformin dosing study |
| Sample size | not specified in source animals verification: verified_2026-07-11 |
| Exposure | 0.1 (lifespan-extending) / 1 (toxic) % metformin in diet — Chronic dietary exposure; lifespan follow-up verification: verified_2026-07-11 |
| Comparator | Control diet |
| Duration | Lifespan study |
| Endpoint | Median lifespan, dose-dependent |
| Evidence tier | Tier 6 · Single-lab animal lifespan |
| What was NOT established | Did not test humans; does not establish a safe or effective human dose by extrapolation. Non-monotonic dose-response means higher doses are not assumed safer or more effective. |
Result values (as reported in sourcing brief)
| low dose result | +~5.83% median lifespan (male mice) at 0.1% dietary dose |
| high dose result | 1% dietary dose was TOXIC (net negative) |
| interpretation | Non-monotonic dose-response: benefit at low dose, toxicity at higher dose |
| verification | verification: verified_2026-07-11 |
Source citation
Verification status: verified This record was checked against its primary publication on 2026-07-11.Identifiers: PMID verified; DOI verified; PMC not reported; trial registry ID not applicable. Record-level verification and identifier-level status are separate: a record can be fully verified against its primary publication while an identifier that does not apply to the study type — a trial registry ID for an animal study, for instance — is marked not applicable. No identifier is fabricated, and none is left in a “pending” state.
| program | Metformin mouse lifespan dosing research (Barzilai/de Cabo/AFAR-associated program) |
| reported year | not recorded |
| pmid | 23900241 verified |
| doi | 10.1038/ncomms3192 verified |
| journal | Nature Communications 2013;4:2192 |
| authors | Martin-Montalvo A, Mercken EM, Mitchell SJ, et al. (de Cabo lab) |
| year | 2013 |
| verified date | 2026-07-11 |
| verified note | 0.1% w/w dietary metformin from middle age extended male-mouse lifespan + healthspan (AMPK up, oxidative damage down); 1% w/w TOXIC/shortened survival — MATCHES. Exact +~5.83% median to be read from survival data. |
| correction | Record ID says 'itp' but this is Martin-Montalvo/NIA (de Cabo), NOT an Interventions Testing Program cohort. Rename record metformin-mouse (not -itp). |
| verification status | verified |
Funding / conflict of interest
not compiled in this proof-of-concept verification: verified_2026-07-11
Version 0.2.0-mvp · literature search date 2026-07-11 · editorial owner Nabus Research