Mus musculus (UM-HET3, genetically heterogeneous) — Multi-site NIA ITP cohort (Jackson Laboratory, University of Michigan, University of Texas sites)   Tier 5 · Replicated animal lifespan verification: verified
1. Species / populationGenetically heterogeneous UM-HET3 mice, 3 NIA ITP test sites
2. Exposure, route, schedule~14 ppm in chow, begun at ~600 days of age (late-life start)
3. Comparator & durationControl chow, same-age cohort; followed to natural death
4. Endpoint & numeric resultAge at 90% mortality +14% (female) / +9% (male) at ~14 ppm in chow; replicated at 3 independent ITP sites
5. What this study did NOT establishNo human lifespan data; no dosing-equivalence or safety data for human 'longevity' use
Intervention(s)Rapamycin
SpeciesMus musculus (UM-HET3, genetically heterogeneous)
PopulationMulti-site NIA ITP cohort (Jackson Laboratory, University of Michigan, University of Texas sites)
Sample sizenot specified in source animals verification: verified_2026-07-11
Exposure~14 ppm in chow — Initiated at ~600 days of age (late-life start) verification: verified_2026-07-11
ComparatorControl chow, same-age cohort
DurationFollowed to natural death (lifespan study)
EndpointAge at 90% mortality (90th-percentile lifespan); median lifespan
Evidence tierTier 5 · Replicated animal lifespan
What was NOT establishedDid not test human lifespan, human dose equivalence, or safety of any 'longevity dose' in humans. Late-life initiation in mice does not establish outcomes for earlier-life human initiation. Does not establish an optimal or safe rapamycin dosing schedule for humans.
metricage at 90% mortality (90th-percentile lifespan)
female change at 90pct mortality+14%
male change at 90pct mortality+9%
replicationReplicated across 3 independent ITP test sites
separation noteThis record is the 2009 single-dose report at ~14 ppm only. The 42-ppm dose-response figures are a separate 2014 study (Miller et al., Aging Cell) and live on itp-doseresponse-rapamycin. The two also measure different quantities: 90%-mortality age here, median lifespan there.
verificationverification: verified_2026-07-11
Verification status: verified This record was checked against its primary publication on 2026-07-11. PubMed/PMC/Lancet lookup; numbers cross-checked against abstract.Identifiers: PMID verified; DOI verified; PMC verified; trial registry ID not applicable. Record-level verification and identifier-level status are separate: a record can be fully verified against its primary publication while an identifier that does not apply to the study type — a trial registry ID for an animal study, for instance — is marked not applicable. No identifier is fabricated, and none is left in a “pending” state.
programNIA Interventions Testing Program (ITP)
reported year2009
pmid19587680 verified
doi10.1038/nature08221 verified
journalNature
pmcPMC2786175 verified
authorsHarrison DE, Strong R, Sharp ZD, et al. (NIA ITP)
year2009
verified date2026-07-11
verified methodPubMed/PMC/Lancet lookup; numbers cross-checked against abstract
verified note+14% female / +9% male lifespan at 90%-mortality age, ~14 ppm (14.7 mg/kg food), initiated 600 days, replicated 3 sites — MATCHES abstract.
correctionDose-response arm (4.7/14/42 ppm) belongs to the SEPARATE 2014 Miller study (itp-doseresponse-rapamycin), not this 2009 single-dose report; do not attribute 42-ppm figures to Harrison 2009.
verification statusverified
reverified 20260808DOI re-confirmed against Crossref 2026-08-08 (Nature 460:392-395, author list matches).

Funding / conflict of interest

not compiled in this proof-of-concept verification: verified_2026-07-11

Version 0.1.0-poc · literature search date 2026-07-11 · editorial owner Nabus Research