Study record
Solon-Biet nutritional geometric framework (25-diet mouse study)
Median lifespan; circulating IGF-1
Mus musculus — Mice, 25 distinct diets varying protein:carbohydrate:fat ratios (nutritional geometry design) Tier 6 · Single-lab animal lifespan verification: verified
1. Species / populationMice, Solon-Biet et al. nutritional-geometry study, 25 diets
2. Exposure, route, scheduleGeometric range of dietary protein:carbohydrate ratios, chronic exposure
3. Comparator & durationCross-diet comparison; lifespan follow-up
4. Endpoint & numeric resultLongest lifespan on low-protein/high-carbohydrate diets; lower IGF-1 associated
5. What this study did NOT establishNo human data; IGF-1 association not established as causal; no isoleucine- or BCAA-specific numeric breakdown
Full record
| Intervention(s) | Protein Restriction, BCAA Restriction (Branched-Chain Amino Acid Restriction), Isoleucine Restriction |
| Species | Mus musculus |
| Population | Mice, 25 distinct diets varying protein:carbohydrate:fat ratios (nutritional geometry design) |
| Sample size | not specified in source animals verification: verified_2026-07-11 |
| Exposure | 25 diets geometric range of protein:carbohydrate ratios — Chronic dietary exposure; lifespan follow-up verification: verified_2026-07-11 |
| Comparator | Cross-diet comparison within the same nutritional-geometry framework |
| Duration | Lifespan study |
| Endpoint | Median lifespan; circulating IGF-1 |
| Evidence tier | Tier 6 · Single-lab animal lifespan |
| What was NOT established | Did not test humans. Association between low protein/high carbohydrate and lower IGF-1 is not established as causal for the lifespan difference. Does not supply an isoleucine-specific or BCAA-specific numeric effect distinct from total-protein manipulation. |
Result values (as reported in sourcing brief)
| longest lifespan diet | Low-protein, high-carbohydrate diets |
| igf1 association | Lower circulating IGF-1 associated with the longest-lived diet groups |
| verification | verification: verified_2026-07-11 |
Source citation
Verification status: verified This record was checked against its primary publication on 2026-07-11.Identifiers: PMID verified; DOI verified; PMC not reported; trial registry ID not applicable. Record-level verification and identifier-level status are separate: a record can be fully verified against its primary publication while an identifier that does not apply to the study type — a trial registry ID for an animal study, for instance — is marked not applicable. No identifier is fabricated, and none is left in a “pending” state.
| program | Solon-Biet/Simpson/Raubenheimer nutritional geometry research program |
| reported year | not recorded |
| pmid | 24606899 verified |
| doi | 10.1016/j.cmet.2014.02.009 verified |
| journal | Cell Metabolism 2014;19:418-430 |
| authors | Solon-Biet SM, McMahon AC, Ballard JWO, et al. |
| year | 2014 |
| verified date | 2026-07-11 |
| verified note | 25 ad-libitum-diets geometric framework; low-protein/high-carbohydrate (LPHC) maximized median lifespan; reduced total energy had no positive longevity effect; LPHC lowered mTOR/IGF-1-related signaling — MATCHES. |
| verification status | verified |
Funding / conflict of interest
not compiled in this proof-of-concept verification: verified_2026-07-11
Version 0.2.0-mvp · literature search date 2026-07-11 · editorial owner Nabus Research